Studi eksperimen isolasi fukoidan dari rumput laut sargassum binderi sonder: Efek suhu dan waktu ekstraksi

Herti Utami; Zulaikha Setya Mega Sari; Muhammad Hanif; Yuli Darni; Simparmin br Ginting; Elida Purba

doi:10.36706/jtk.v29i2.1462

Herti Utami Jurusan Teknik Kimia, Fakultas Teknik, Universitas Lampung, Bandar Lampung – Indonesia
Zulaikha Setya Mega Sari Jurusan Teknik Kimia, Fakultas Teknik, Universitas Lampung, Bandar Lampung – Indonesia
Muhammad Hanif Jurusan Teknik Kimia, Fakultas Teknik, Universitas Lampung, Bandar Lampung – Indonesia; Department of Chemical Engineering, National Tsing Hua University, Hsinchu City – Taiwan
Yuli Darni Jurusan Teknik Kimia, Fakultas Teknik, Universitas Lampung, Bandar Lampung – Indonesia
Simparmin br Ginting Jurusan Teknik Kimia, Fakultas Teknik, Universitas Lampung, Bandar Lampung – Indonesia
Elida Purba Jurusan Teknik Kimia, Fakultas Teknik, Universitas Lampung, Bandar Lampung – Indonesia

DOI: https://doi.org/10.36706/jtk.v29i2.1462

Keywords: fukoidan, rumput laut coklat, crude fucoidan, asam etilen diamin tetra asetat

Abstract

Fukoidan adalah senyawa bioaktif yang merupakan polisakarida sulfat dengan penyusun utamanya yaitu L-fukosa dan sulfat. Fukoidan ini terdapat pada rumput laut coklat jenis Sargassum binderi Sonder. Fukoidan berfungsi sebagai antikoagulan, antioksidan, antikomplementari, anti pembengkakan, pelindung lambung, anti kanker, immunostimulan dan pengatur kadar lipid darah. Terdapat berbagai pelarut untuk mengekstrak fukoidan dari rumput laut coklat, diantaranya memakai pelarut etanol, HCl, air, CaCl₂, dan asam etilen diamin tetra asetat. Tujuan dari penelitian ini adalah mendapatkan pengaruh suhu serta waktu ekstraksi dengan pelarut asam etilen diamin tetra asetat dalam ekstraksi rumput laut coklat jenis Sargasum binderi Sonder. Proses ekstraksi dilakukan pada suhu 50 ^oC, 60 ^oC, 70 ^oC, dan 90 ^oC, dan waktu 1 jam, 2 jam, 3 jam, 4 jam dan 5 jam. Hasil penelitian menunjukkan bahwa yield crude fucoidan tertinggi yaitu pada suhu 90 ^oC dan waktu 2 jam sebesar 8,19 %, namun yield tertinggi dengan karakteristik terbaik pada suhu 70 ^oC dan waktu 2 jam sebesar 7,98 %. Berdasarkan hasil analisis didapatkan total kandungan gula sebesar 38,54 % dan kandungan sulfat sebanyak 13,91 %.

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