Validasi proses kristalisasi dekstrosa monohidrat kualitas mikrobiologi sistem batch pada skala bench
Abstract
Validasi proses kristalisasi telah dilakukan untuk produksi Dekstrosa Monohidrat (DMH) kualitas mikrobiologi yang mempunyai kemurnian tinggi secara sistem batch pada skala bench kapasitas 3 - 4 kg produk. DMH tersebut banyak digunakan sebagai bahan kimia di laboratorium dan industri khususnya di bidang mikrobiologi, bioteknologi dan biofarmasi. Penelitian ini bertujuan untuk memvalidasi proses kristalisasi DMH kualitas mikrobiologi secara sistem batch. Validasi proses yang dilakukan adalah meliputi tahapan pelarutan bahan baku, kristalisasi, sentrifugasi dan pengeringan serta analisis produk. Dalam penelitian ini, proses kristalisasi dilakukan dengan parameter profil penurunan suhu secara linier, penambahan seed sebanyak 0,5% , kecepatan pengadukan 50 rpm dan waktu kristalisasi 72 jam. Hasil validasi proses produksi DMH kualitas mikrobiologi telah dilakukan sebanyak 3 kali ulangan dengan yield masing-masing adalah 54,62%, 55,66% dan 56,85%. Parameter kualitas produk ditunjukkan oleh kemurnian DMH (HPLC Area %) masing-masing adalah 99,53%, 99,61% dan 99,15 % serta parameter lainnya yang sudah memenuhi persyaratan sesuai standar produk yang ada di pasar.
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